Re: Problems with matrix deposition

[Bob]

Thanks a lot for your advice Pierre. 😀

I have been discussing the matter with the guys who performed the sectioning for me. It looks like they had been sectioning the sample at room temp rather than cryogenically freezing the sample first. Would I be right in thinking that you will lyse more cells (and hence obtain more protein/peptides on the tissue surface for imaging) if the section is performed on a frozen sample and could this perhaps be the cause of my problems rather than the thickness? Or should the temperature not make much difference?

Any further help would be much appreciated! 😉

Bob