Re: Problems with matrix deposition

[chaurap]

Bob,

Most of the tissue profiling and imaging work has been performed on thin sections cut from fresh frozen samples. I recommend reading the two references below for protocols.

Direct tissue analysis using matrix-assisted laser desorption/ionization mass spectrometry: practical aspects of sample preparation. Schwartz SA et al, J. Mass Spectrom. (2003) 38: 699-708.

Integrating histology and imaging mass spectrometry. Anal. Chem. Chaurand P. et Al, (2004) 76(4): 1145-1155.

You can also work with fresh (non-frozen) tissues. In this case, one approach is to blot the protein on hydrophobic surfaces. Please refer to:

Direct Profiling of Proteins in Biological Tissue Sections by MALDI Mass Spectrometry. Chaurand P. et Al, (1999) 71(23): 5263-5270.

If you need the pdf’s, I can send them to you.
Good luck!
Pierre