Front Page Forums BioMap Histogram function in BioMap Re:Histogram function in BioMap

Ramin Vismeh

Dodge and Markus,

Thanks. So you guys think that the intensities from ROI regions CAN be used as a base for quantification as they are not cut or scaled?

WhenI say the intensities do not match, I am talking about the average. When I average my raw data (say for the whole image) for a specific m/z, teh average is most of the times more than 32767! But if I select the whole image as one ROI, teh intensity for that specific m/z would not be the same in the ROI intensity panel! Is’nt the intensity divided by the number of pixels for the ROIs? another words: the ion intensity that I get from Analysys—>Plot—>ROI is the ion intensity/pixel?