Re:Ion intensity for ROI

[Ramin Vismeh]

Dave,

I am using DESI. So, my spot size is not known actually. I can have an estimation but it wont be accurate. Thats why I am trying to assess the area using the image. How do you usually generate the standard curves? I assume you use something like ion intensity vs. ng/area for some spots of standard that you put on tissues. Is that right? When you image the tissue, well most of the times the compound you are looking for is not probably in one nice circle spot. It is usually scattered all over the tissue. So, I assume for quantification the right unit to use is ng/area. Am I right?

Thanks