appearance size of img files

[klois]

i converted a bruker file to ANALYZE 7.5 format, for multiple m/z values (as values on z axis).
however, converting a file for only one m/z value, the image fits into the display area perfectly, while the more m/z values i put into one image file, the smaller it appears (but the dimension is the same – just more z values).

strange… is there something i should change/look at in the header file?

thx
klois

[Markus Stoeckli]

It’s not clear to me what you try to achieve and where it goes wrong ❓ Could you post an example?

markus

[Anonymous]

well, it’s not that there’s really something wrong.

we have raw data from a bruker mass-spec for some 50×50 points and for each 80.000 values.
for the points we take x and y, and for the value the z ( for less then 2^15 – 1 values ) axis.
if we only compute the *.img file for one m/z value ( so z=1, 2-dimensional picture), the picture fits perfectly into the biomap window.

if we compute the pictures of more m/z values, ( eg z = 256 ), the computed *.img file has to be viewed at a higher zoom factor ( eg 16 ) to fit into the biomap window.
the image resolution remains the same, you just have to use the zoom, to make biomap display it in the same size inside the window.

example *.img file is rather big, so i hope the problem is clearer now…

thx
martin

[Markus Stoeckli]

I assume that you use the z-dimension to save multiple selected images? Or you stitch them together as tiles? When we save our data, we put the spectra information in the 4th dimension which allows to use all the BioMap functions.

Markus

[klois]

Thanks, that solved our problem!

[Author]

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