Data analysis using Global Scans and Analysis Mode

[Cynthia McNees]

Hello, I have a couple of questions about the analysis of images using Biomap

When using Biomap to analyze my images, what is the software doing to the raw data when 0-100 scans of approximately 32,000 scans are selected using the Analysis-Global-Scan to generate the m/z spectra? Also, data when selecting a particle m/z range within the spectra for export to another window using spectral analysis, what is the software doing to the raw data and how do each of the options for Analysis Mode adjust the raw data?

Thanks in advance for your help.

Ruth

[Markus Stoeckli]

When you click on Analyis – Plot – Global – Scan you are presented with a window where you enter a Maximum and Minimum intensity. So, in your case where you selected 100 and 0, all the spectra which fall into this intensity range are averaged. A typical use is to exclude low signal spectra and saturated spectra.

By selecting a m/z range in the spectrum you get multiple options on what the software should do: Mean, Max, Mean with BC, Max with BC and Peak Shift. Please be aware, that all these functions work with the raw data and that there is no peak fitting involved. The result is just the mathematics on the selected data points as indicated by the name. Do determine the baseline, we take the average of 3 points on either side of the selected range.

Markus

[Author]

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