After imaging several rat kidneys I find that one section usually the lower half to third of the kidney has much higher intensity in biomap. Does anyone have any idea what this maybe?
By lower do you mean the section that is run earlier? It might be matrix evaporation. In long runs (> 2hrs) matrix begins to sublimate this gives a “graded” intensity shift. It happen to us when we were using 2,6 DHA.
We sometime observe a sudden shift in intensity, making a “break” in intensity i.e. a bottom half is lighter than the top half for example. We can’t proof it, but we think it’s an instrument problem.
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